The Big Top in Waddesdon, which opens on Monday, will be open 51 weeks of the year taking children from as young as three-months through to four-years-old before they start school.
Manager Sylvia Robinson said: “We are the only nursery within the village and within a five mile radius.
“We look forward to becoming a part of the community here.”
The nursery is based in a former office block which has been empty for two years.
Mrs Robinson said: “We’ve given it a new lease of life.”