Thieves have ransacked Aylesbury’s St Mary’s Church, taking cash and causing damage throughout the building.
Around £165 was taken from a cash tin and burglars rifled through the rest of the church looking for valuables.
The raid left much of the church in a mess, but staff have kept it open throughout.
It is the second time burglars have targeted St Mary’s this year.
Parish administrator Karen Baker said: “They must be very desperate to do this.
“It’s very sad that they’re in that state that they need to do such a thing.
“There was more money in the tin than usual because we’re having a wedding blessing this weekend so we had money for the champagne.
“They have rifled through all of our offices and pulled everything out.
“Everything valuable is locked away, fortunately. It’s the damage and upheaval that’s the main thing.”
The burglary is believed to have taken place between 7pm on Sunday and 7am on Monday.
It is thought the thieves broke in through one of the doors.
Staff set up an office on the church’s grand piano while forensic officers were at the scene.
Father Shane Wood tweeted: “Thank you for prayers over church being broken into.
“The Dunkirk spirit is setting in after many sad people turning up for work at church.
“Thanks to a great set of staff coping with all the chaos.”
Anyone with information should call police on 101 or Crimestoppers anonymously on 0800 555 111.