Work has already started on the unit at Broadfields Retail Park and it should be welcoming diners by September.
The restaurant is replacing Pizza Hut, which is now closed.
The chain is looking to recruit and train up to 40 new team members and the job opportunities will be advertised locally shortly before opening.
A spokesman said: “Harvester is the perfect place to bring food, family and friends together and Broadfield Retail Park offers us a great opportunity to bring this to Aylesbury.”
Meanwhile, it is understood that PC World and Currys – which are both owned by the Dixon group – are set to move into a single unit together at Broadfields.
However, a Dixons spokesman said: “We have a nationwide programme of refitting and, in certain instances, relocating Currys and PC World stores to make them easier and more exciting places for customers to shop.
“We are considering various options in the Aylesbury area but there is no further update at this time.”